Molecular identification and characterization of natural fruit pulp in reconstituted juices in Kenya/ (Record no. 88546)

MARC details
000 -LEADER
fixed length control field 03678nam a22001937a 4500
003 - CONTROL NUMBER IDENTIFIER
control field KE-MeUCS
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20240429165546.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 240411b xxu||||| |||| 00| 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 000000
040 ## - CATALOGING SOURCE
Transcribing agency KE-MeUCS
Modifying agency KE-MeUCS
050 ## - LIBRARY OF CONGRESS CALL NUMBER
Classification number QH506.T66 2023
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Tombito Collins Khagali
245 ## - TITLE STATEMENT
Title Molecular identification and characterization of natural fruit pulp in reconstituted juices in Kenya/
Statement of responsibility, etc Collins Khagali Tombito
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc Meru
Name of publisher, distributor, etc Collins Khagali Tombito
Date of publication, distribution, etc 2023
300 ## - PHYSICAL DESCRIPTION
Extent xii, 99p
500 ## - GENERAL NOTE
General note A thesis submitted in partial fulfillment of requirements for conferment of the Degree of Masters of Science in Molecular Biology of Meru University of Science and Technology
520 ## - SUMMARY, ETC.
Summary, etc Fruits and fruit-based products comprise abundant bioactive compounds valuable to human health <br/>and may reduce the risk of disease by beneficially targeting body functions. Consequently, these <br/>fruits are used in varying proportions as ingredients in functional foods. The market sectors for <br/>fruit juices have been growing at a fast pace. The widening market of these products has led to <br/>speculation that they may contain artificial aromas, adulterated and mislabeled. Fruits are <br/>relatively easy to authenticate morphologically when intact and fresh. However, the act of <br/>processing them into juice gives rise to the possibility of substitution with cheaper products. For <br/>this reason, processed food product authentication is primarily significant for consumers; <br/>industries, and regulatory agencies. Effective, reliable, and rapid food authentication methods are <br/>valuable tools for the identification of natural fruit pulp in reconstituted fruit juices to ensure juice <br/>quality and safety hence mitigating adulteration and fraud. Molecular-based methods have recently <br/>acquired immense priority for their ability to pick food material sources at any stage along the food <br/>supply chain. The study focused on DNA isolation from raw and reconstituted fruit juices. The <br/>study aimed to validate an appropriate DNA isolation protocol specifically for processed fruit <br/>juices. It describes an innovative experimental methodology that efficiently extracts, amplifies, <br/>and identifies natural fruit juice pulp by utilizing universal biomarkers to test for the quality and <br/>authenticity of natural fruit pulps in reconstituted fruit juices in Kenyan markets. Two genomic <br/>DNA extraction protocols; CTAB and SDS were tested for the isolation of DNA from processed <br/>fruit juices. The CTAB and SDS methods were able to recover genomic DNA of high quality and <br/>purity appropriate for application in various PCR analyses with few limitations in the CTAB <br/>protocol. The concentration of the DNA was determined using the Nano-drop spectrophotometer <br/>in ng/μL by calculating the absorbance at wavelengths (A260/A280: A260/A230). The quality of <br/>the extracted DNA was evaluated on 0.8% agarose gel electrophoresis stained with lul ethidium <br/>bromide and observation of bands integrity was done in a UV-trans-illuminator machine (Quantum <br/>ST4, France). PCR amplification was done using universal primers (rbcL-650 bp, psbA-323 bp) <br/>that target the plant chloroplast genome). DNA extracted from the SDS method exhibited <br/>robustness and ease during the PCR amplification process. The amplified bands' quality and <br/>integrity were evaluated on 1.5% agarose gel stained with 1 ng/L ethidium bromide. From the <br/>results obtained, the SDS protocol emerged as the best for extracting high-quantity and amplifiable <br/>DNA. <br/>KEYWORDS; <br/>Adulteration, Molecular markers, Fruit Juice, Food Safety, Quality, Protocol, DNA, SDS, CTAB
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Thesis
Cataloguer Intern
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Source of acquisition Cost, normal purchase price Cataloger Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
    Library of Congress Classification     Meru University Meru University Periodical Section 11/04/2024 - 0.00 Intern   QH506.T66 2023 24-37884 11/04/2024 11/04/2024 Thesis

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